Biography:

Dr. A Adetutu is affiliated to Department of biochemistry, Ladoke Akintola University of Technology, where Dr. A Adetutu is currently working as Faculty. Dr. A Adetutu has authored and co-authored several national and international publications and also working as a reviewer for reputed professional journals. Dr. A Adetutu is having an active association with different societies and academies around the world. Dr. A Adetutu made his mark in the scientific community with the contributions and widely recognition from honourable subject experts around the world. Dr. A Adetutu has received several awards for the contributions to the scientific community. Dr. A Adetutu major research interest involves Antioxidant, Anticancer, Toxicology and Oncology.

 

Abstract:

The development of resistance to currently known conventional anti-typhoid drugs has necessitated search into cheap, more potent and less toxic anti-typhoid drugs of plant origin. Therefore, this study investigated the anti-typhoid activity of fractions of A. indica in Salmonella typhi infected rats. Leaves of A. indica were extracted in methanol and fractionated into n-hexane, chloroform, ethyl-acetate and aqueous fractions. The anti-salmonella potentials of fractions of A. indica were assessed via in-vitro inhibition of S. typhi using agar well diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and biofilm assays. The biochemical and haematological parameters were determined by spectrophotometric methods. The histological analysis was performed using Haematoxylin and Eosin staining methods. Data analysis was performed by one -way ANOVA. Results of this study showed that S. typhi was sensitive to aqueous and chloroform fractions of A. indica and the fractions showed biofilm inhibition at concentrations of 12.50, 1.562 and 0.39 mg/mL. In the in-vivo study, the extract and chloroform fraction had significant (p<0.05) effects on the number of viable S. typhi recovered from the blood and stopped salmonellosis after 6 days of treatment of rats at 500 mg/kg b.w. Treatments of infected rats with chloroform and aqueous fractions of A. indica normalized the haematological parameters in the animals. Similarly, treatment with fractions of the plants sustained a normal antioxidant status when compared with the normal control group. Chloroform and ethyl-acetate fractions of A. indica reversed the liver and intestinal degeneration induced by S. typhi infection in rats. The present investigation indicated that the aqueous and chloroform fractions of A. indica showed the potential to provide an effective treatment for salmonellosis, including typhoid fever. The results of the study may justify the ethno-medicinal use of the extract in traditional medicine for the treatment of typhoid and salmonella infections

Biography:

Sher Ali has completed his BSc in Microbiology from the Faculty of Health Sciences, Hazara University, Mansehra, Pakistan in 2011. After completion of his degree, he has joined the SRSP (Sarhad Rural Support Program) as a Health Promotion and Research Officer.

 

Abstract:

The study was carried out in Hazara University, Mansehra, Pakistan in 2011 on the antimicrobial activity of honey against specific microbes which include like E. coli, Salmonella, Staphylococcus aureus, Enterococcus faecalis and Candida albican. During the study the 37 honey samples were collected from different district of Hazara division and Malakand division like Mansehra Swat and Dir were selected for the samples collection. For the samples collection process 170 indoor and outdoor patients were visited. The microorganisms were isolated from the various samples collected from the patients having the confirmed infection which were further processed in the microbiology laboratory by using nutrient agar incubated on 37 oC for 24 hours. During the research work agar well plate technique were used to examine the maximum zone of inhibition on Muller Hinton agar against all the specified organisms. Result showed that E. coli showed 66 mm, Salmonella Typhi 62 mm, Enterococcus faecalis 60 mm, Candida albican 50 mm and Staphylococcus aureus 38 mm. From the study it is concluded that honey is used against different diseases and infections like wound infection, diarrhea, dehydration, paralysis, enterococcus faecalis, chest infection, jaundice, tuberculosis and Urinary tract infections.

 

Biography:

Abstract:

Pregnant women have always used phytotherapy to treat some ailments during their pregnancy. An Ethnopharmaological approach was used in Tlemcen city (extreme western area of Algeria) to know the profile of the pregnant women who use the medicinal plants to treat their health problems, and to identify and repertory these plants. The survey was conducted among 55 pregnant women interviewed in the university hospital of Tlemcen, using a questionnaire. SPSS was used for statistical analysis.

A total of 46 medicinal plants were reported, belonging to 23 different families.  The majority (34%) of the pregnant women who use plants were young (32,72%) with a university level. Whether they live in or around the city, there is almost no difference in use (27,27% , 29,09 %). The results provide a very valuable source of information about the medicinal flora of the region. It could be a database for other research in the futur to explore the composition of these plants wich are effective against different pregnancy ailments.

 

Biography:

Amani Abdel-latif has completed her PhD 1996 at the through a DAAD scolarschip in Mainz University, Germany. She had postdoctoral Scholarships to do postdoctoral researches at the Institute of Ecophysiology, University of Tübingen, Germany and the institute of Wood Physiology , Hamburg , Germany for 4 months (every year), This work was financed by a grant from the Commission of the European Communities by the Programme Agriculture and Fisheries (contract FAIR CT96 – 1887). She has published more than 20 papers in reputed journals (e.g. Plant methods) and has been serving as Professor of plant physiology at the faculty of science Alexandria university, Egypt.

 

Abstract:

The effect of applying Ulva lactuca aqueous extract (ULAE) with different concentrations on the growth of maize seedlings was studied. The extract was applied as foliar spray and in other experiment was incorporated in the growth nutrient solution. Growth parameters, mineral nutrients, photosynthetic pigments, protein profile, and phospho enolpyruvate (PEPCase) activity were monitored. Presoaking maize grains in different ULAE was an effective technique to obtain better growth. Seedlings sprayed with 0.5% or 1% ULAE showed a significant increase in growth and biochemical parameters. Higher concentration (5%) showed inhibitory effect. There were variable peptides with different low molecular weights that were synthesized in all treatments except for the control one (100% H). For example, in seedlings sprayed with 5% ULAE proteins as 27, 24, 23, 12 and 10 KDa were synthesized. Similar low molecular weight proteins were synthesized in all other treatments but with different band intensities. Furthermore, treatments with ULAE as a foliar spray or supplemented in the growth medium differentially affected protein expression in Z. mays seedlings. New proteins were expressed in the treated seedlings which could be due to the action of components in the extract that are bioactive for growth. PEP carboxylase activity in Z. mays seedlings grown in different combinations of Hoagland’s solution and ULAE was higher than that of seedlings sprayed with ULAE alone. There was a gradual decrease in enzyme activity in response to increasing ULAE applied as foliar spray and the least activity was recorded in seedlings sprayed with 5% ULAE.

 

Biography:

Sainath Sharad Kasar has completed his PhD at the age of 29 years from K.B. C. North Maharashtra University, Jalgaon, Maharashtra, India. He recieved Newton Bhabha Fellowship to complete pre-doctoral tresearch training in Newcastle University, Inited Kingsom for four month.He has published more than 3 papers in reputed international journals.

 

Abstract:

A glycoprotein alpha amylase inhibitor (α-AI), molecular weight of 8.3 kDa, was isolated and purified from seeds of Withania somnifera (WSAI), an important indigenous medicinal plant. It is thermostable and non-competitive type inhibitor of fungal amylase. Mass spectrometric analysis revealed that it shares 59% sequence similarity with Wrightide II type α-AI from W. religiosa. When the adults of T. castaneum were fed with WSAI (1.6 mg/g), decrease in consumption, growth and efficiency of conversion of ingested food were evident along with over 4-fold increase in feeding deterrence index and decrease in longevity. In another approach, the treatment of WSAI to potato slices at 200 ppm concentration for 30 min was found to reduce the browning (60%), residual amylase and polyphenol oxidase activities (~40%) and reduction in reducing sugar level by 25% over control. In conclusion, i) WSAI has good potential in mitigating overall growth and development of T. castaneum and thus make it a potential candidate for its management, individually or in combination with other insect controlling biological molecules and ii) WSAI treatment results in reduction in browning and generation of acrylamide in potato during processing. Thus, it offers a heathier (better nutritional value) and economical (significant improvement in dry weight post frying) alternative method without compromising the overall sensory quality of the finished product.

 

Biography:

Ryan Jay G. Mostoles has completed his Bachelor’s degree in Medical Laboratory Science from Lorma Colleges and  currently taking up his Master’s degree in Biology from Don Mariano Marcos Memorial State University. He is working as Instructor and faculty member of the College of Medical Laboratory Science, Lorma Colleges. He holds the designation as Research Coordinator.

 

Abstract:

The study determined the potential antibacterial property of Karmay leaf extract as an organic component of hand sanitizer using the different concentrations (100%, 75% and 50%) prepared. Specifically, the experiment tried to ascertain which phytochemical constituents of Phyllantus acidus exhibit antibacterial property. It also sought which extract and what percentage concentration exhibits the minimum zone of inhibition against Staphylococcus aureus.The findings of the study revealed that the

(1) Phyllantus acidus contains phenols and tannins which are phytochemical that exhibit antibacterial activity.

(2) the minimum zone of inhibition (5.06 mm) was exhibited on the 100% concentration.

(3) there is no significant difference among the 50%, 75% and 100% concentration with and without preservatives of extracts in terms of their effectiveness against S. aureus.

(4) there is a significant difference between the control and three extracts 50%, 75%, 100%. Based on the findings, the karmay leaf extract does not possess the potential to be an organic component of hand sanitizer.

 

Biography:

Sara Carpi has completed her Ph.D. in Medical Pathophysiology and Pharmacology in  2015. During her post-doc fellowship, she awarded with research fellowships of Italian Pharmacology Society and Merck & Co. She spent a period of study in the ImmunoViroTherpay Lab, Department of Pharmacy of the University of Helsinki (Finland), supported by an EMBO fellowship. She is a researcher in Pharmacology at the Department of Pharmacy, University of Pisa (Italy) from 2017. Her current research is focused on non-coding RNA as mediators of the activity of natural compounds

Abstract:

Extra-virgin olive oil (EVOO) polyphenols contribute to Mediterranean diet health-promoting properties. One of the most abundant secoiridoid present in EVOO, Oleacein (OA), demonstrated anticancer activity against several tumors. Nevertheless, its role against melanoma has not still investigated. This study aimed at determining in vitro the anti-melanoma activity of OA and the relative mechanism of action. OA induced cell growth inhibition in 501Mel melanoma cells with an IC50 in the low micromolar range of concentrations. Moreover, an OA concentration approximating the IC50 induced G1/S phase arrest, DNA fragmentation, and down-regulation of genes encoding anti-apoptotic (BCL2 and MCL1) and pro-proliferative (c-KIT, K-RAS, PIK3R3, mTOR) proteins, while increased transcription levels of the pro-apoptotic protein BAX. Concordantly, OA increased the levels of miR-193a-3p (targeting MCL1, c-KIT and K-RAS), miR-193a-5p (targeting PIK3R3 and mTOR), miR-34a-5p (targeting BCL2 and c-KIT) and miR-16-5p (targeting BCL2, MCL1, K-RAS and mTOR), while decreased miR-214-3p (targeting BAX). These modulatory effects might contribute to the inhibition of 501Mel melanoma cell growth observed after treatment with an olive leaves- derived formulation rich in OA, with potential application against in situ cutaneous melanoma. Altogether, these results demonstrate the ability of OA to contrast the proliferation of cutaneous melanoma cells through the transcriptional modulation of relevant genes and microRNAs, confirming the anticancer potential of EVOO and suggesting OA as a chemopreventive agent for cancer disease therapy.