Ehssan Hassn Osman Moglad
The National Center for Research, Sudan
Title: Anticancer potential of various extracts and fractions of Solanum nigrum
Biography
Biography: Ehssan Hassn Osman Moglad
Abstract
Solanum nigrum L. (Solanaceae) is locally called as Enab el Deib. Generally, in Sudan has been known to be used as folkloric medicine for cancer treatment. In the present work, we have aimed to investigate in vitro anticancer activity of eighty percent methanol and chloroform extracts of leaves and stems of Solanum nigrum using PC-3 human prostate cancer cell lines and Hela cervical cancer cells by MTT assay. All extracts in concentration 100 µg/ml showed anticancer activity in PC-3 and Hela and the highest percentage of growth inhibition obtained from stems methanol extracts on Hela 91.11% followed by leaves and stems methanol extracts (74.28 and 80.49 respectively) on PC-3. Leaves and stems of methanol extracts showed high growth inhibition percentages of cancer cell. These extracts were fractionated by using three solvent; Acetyl acetate, n- Butanol and Aqueous. Fractions were tested for their anticancer activity on PC3 prostate cancer cell line and Hela cervical cancer cell line using different fraction concentrations i.e. 50, 25 and 5µg/ml by MTT assay. The results obtained revealed that the most active fraction was the leaves n-Butanol fraction that inhibited both type of cancer cells with growth inhibition percentage of 99.81±0.49 on PC3 and 98.59±0.21 on Hela cell line with inhibition concentration for 50 percent of cells growth IC50 5.92±0.46 µg/ml and 1.6±0.17 µg/ml respectively. This result supports the traditional use of Solanum nigrum for the treatment of cancer in different regions of the Sudan.