Gan Sook Yee
International Medical University, Malaysia
Title: Cholinesterase inhibitory and protective effects of algal compounds against neurotoxicity and neuroinflammation
Biography
Biography: Gan Sook Yee
Abstract
Fucosterol, pheophytin A and pheophytin A isomer isolated from brown seaweeds such as Padina ornata and Sargassum polycystum as well as caulerpin isolated from the green seaweed, Caulerpa racemosa were assayed for their cholinesterase inhibitory activities and their neuroprotective effects on amyloid β1-42 (Aβ1-42) / glutamate induced SH-SY5Y cells. Their anti-neuroinflammatory effects were also determined by measuring the levels of cytokines and pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated C8-B4 microglia cells. All four algal compounds exhibited inhibitory activities on acetylcholinesterase and butyrylcholinesterase in a dose dependent manner. They significantly increased the viability of Aβ1-42 / glutamate induced SH-SY5Y cells but suppressed the expression of TNF-α, IL-1β, IL-6, NO and PGE2 in LPS-stimulated C8-B4 cells. The four algal compounds showed dual cholinesterase inhibition and neuroprotective effects against Aβ1-42/ glutamate/ LPS suggesting possible applications for the prevention of Alzheimer’s disease.
