Shawky Mohamed Aboelhadid
Beni Suef University, Egypt
Title: Control of sarcoptic mange in rabbits using lemon oil
Biography
Biography: Shawky Mohamed Aboelhadid
Abstract
The toxicity effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water or 70% ethyl alcohol (2.5, 5, 10, 20, 50 & 100%). In vitro application was done in 5 replicates for each concentration. The readings of treatment were recorded at 1, 12 and 24 hours post application (PA). Field trial was done by using 20% lemon oil. Twenty four naturally infected rabbits were divided into 3 groups 8 in each; lemon oil 20% and deltamethrin treated and untreated control groups. The infected parts of rabbits were treated topically once a week for 4 successive weeks. In vitro applications showed that lemon oil 10% & 20% diluted in water caused toxicity to 99.80% and 100.00% after 24 hours PA respectively. Oxidative stress profile in treated mites revealed that treated mite by lemon 20% had significantly (P<0.05) highest hydrogen peroxide and malondialdehyde in compared with mite treated by deltamethrin or distilled water. Otherwise, glutathione peroxidase was significantly (P<0.05) different between treated groups. In vivo application of 20% lemon oil showed complete recovery from clinical signs, absence of mite from microscopic examination from the second week of treatment. In addition, productive performance in lemon treated group was significantly better than infected untreated groups. Also, the treated tissue showed stoppage of scales formation and hair growth faster than deltamethrin treated rabbits. Consequently, lemon oil has remarkable miticidal effect in vitro and in vivo applications.