Irma Antasionasti
Gadjah Mada University, Indonesia
Title: Determination of antioxidant activity and identification of active compounds in avocado peel
Biography
Biography: Irma Antasionasti
Abstract
A research had been conducted to test antioxidant activity and to identify the active compounds of antioxidant in avocado peel. The research aims to determine antioxidant activity of the extract, fractions, and isolates from avocado peel using in vitro method based on DPPH and ABTS radical scavenging, reducing power of iron (III), total phenolic and flavonoid content. To identify the structure of the active compound of antioxidants in avocado peel, FTIR spectroscopy and GC-MS are used. Among extract and fractions evaluated, methanol extract has the antioxidant activity with IC50 values of DPPH, ABTS radical scavenging and reducing power of 9.467±0.045mg/mL, 1.122±0.008mg/mL and 742.863±3.487 mg of ascorbic acid/gram of extract, respectively. Further sub-fractionation revealed that fraction 8 of methanol extract has the most active antioxidant with IC50 values using DPPH, ABTS radical scavenging and reducing power of 4.221±0.137mg/mL, 0.855±0.013mg/mL and 723.067±18.849 mg ascorbic acid/gram fraction, respectively. The relationship between antioxidant activity with the content of total phenolic revealed that the contributions of phenolics contents toward antioxidant activity using methods DPPH radical scavenging, ABTS radical scavenging and reducing power are 37%, 40.78% and 47.45%, respectively. Meanwhile, flavonoid contents contribute to DPPH radical scavenging, ABTS radical scavenging and reducing by 26.71%, 19.25% and 34.62%, respectively. Isolation of the methanol extract (fraction 8) indicated the presence of compound mixture of 1, 2, 4-trihidroksiheptadek-12, 16-diyne and isolation of the ethyl acetate extract obtain 1, 2, 4-trihidroksiheptadek-16-yne-18-ene.
