Anjum Perveen
University of Karachi, Pakistan
Title: In vitro antioxidant potential and free radical scavenging activity of various extracts of pollen of Nelumbo nucifera: Nelumbonaceae
Biography
Biography: Anjum Perveen
Abstract
Ethnopharmacology is the systematic study of substances in plants used to cure common diseases and their relationship with the ethnic/local people. Antioxidant potential of the pollen of Nelumbo nucifera Gaertn using Ferric Reducing Power, Metal Chelating Activity and Trolox Equivalent Antioxidant Capacity (TEAC) assays has been carried out in the current research work. The antioxidant components were initially extracted from the pollen in methanol and were further fractionated in solvents of different polarity such as n-Hexane, Chloroform, Ethyl Acetate and Water. The extract was then employed for ABTS.+ assay to determine free radical scavenging ability, FRAP assay to identify ferric reducing ability and metal chelating ability for its chelating potential. Moreover, to correlate antioxidant ability with its phytochemical composition, the total phenolic and flavonoid contents were also determined. TEAC values ranged from 6.0-9.1 mM of trolox equivalents. Total Phenolic Content values ranged from 5410-8150 mg/l of Gallic Acid. Ferric reducing antioxidant power values for different fractions varied from 49.6-87.5 of FeSO4. Methanol extract which was found to have high reducing power and total phenolic contents has considerable prospective to utilize as a natural antioxidant and be capable to link with the total phenolic contents of plant.