Dawood Ahmed
University of Haripur, Pakistan
Title: Anti-cancer activity of the Taraxacum Officinale L. flowers on rat
Biography
Biography: Dawood Ahmed
Abstract
Objective: Research was conducted to find out the cytoprotective role of T. officinale against the injuries of the tissues induced by carbon tetrachloride.
Material & Methods: Different fractions of the plant were used in vitro and in vivo biological assays. Furthermore, in order to evaluate anti-tumor activity, experimental animals were grouped into 10 batches of 12 male rats in each group. Control group (Group 1), normal water and food were given; and Group II were subjected to dose of chemicals used as a vehicle drugs. Group III all rats of this group (n-12) were injected intraperitoneally with CCl4 (8 mL/kg of rat b.w.) mixed with olive oil in proportion of 0.5:4.5 once a week for 18 weeks. All rats of this group (Group IV) were treated with 50 mg/kg b.w. of rutin (50 mg of rutin in 4 mL of DMSO) as positive control (reference drug) (Group V) were treated by CCl4 (8 mL/kg of animal b.w.) dissolved with olive oil in proportion of 0.5:4.5. After 48 hours, the methanol plant extract and n-hexane extract dissolved in DMSO was given orally once a week for 18 weeks with proportional increase of 50 mg, 100 mg and 200 mg for (Group V), (Group VI), (Group VII), (Group VIII) and for (GroupIX ), (Group X) only plant extracts in methanol and n-hexane.
Results: Histopathological and biochemical study is also evident for the protective effects of T. officinale. CCl4 group showed the severe damages to the tissues and tumor related changes whereas, at the same time, the leaf extracts of T. officinale in methanol and n-hexane prevent the tissue injuries and maintain the normal histological features of the tissue. Biochemical study also reflected the same preventive effects of T. officinale extracts. The protein level was increased, while the activity of