Yongsub Yi
Hoseo University, South Korea
Title: Anti-melanogenesis and anti-inflammatory effects of Opuntia humifusa
Biography
Biography: Yongsub Yi
Abstract
This study was to confirm the effects of anti-melanogenesis and anti-inflammatory effects from Opuntia humifusa fruit and stem extracts. A potent anti-oxidant activity was showed from the leaf extract at IC50 value of 38.33±1.07 μg/mL and fruit extract at IC50 value of 40.23±2.21 μg/mL by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Also, phenolic contents were confirmed through total phenolic assay by high performance liquid chromatography (HPLC). Fraction of taxifolin from leaf extract was identified using HPLC and gas chromatography/mass spectrometry. The extracts of Opuntia humifusa fruit and stem were confirmed about toxicity effect in B16 F1 by cell viability. It was also observed that the melanin contents were decreased. Opuntia humifusa fruit and stem extracts had a positive effect on melanin synthesis inhibition for skin whitening. In investigating the anti-inflammatory activities of Opuntia humifusa, the results of cell viability indicated that taxifolin did not show any cytotoxicity effect on RAW264.7 cells at 500 μM of concentration. The result show that taxifolin inhibited lipopolysaccharide (LPS)-induced production of nitrite oxide (NO). In addition, taxifolin indicated the inhibition of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α and interleukin (IL)-6 productions by cytokine assay and cyclooxygenase (COX)-2 expressions by Western blot analysis, meaning that taxifolin had a significant anti-inflammatory effect. Our results suggested that taxifolin from Opuntia humifusa has anti-melanogenesis and anti-inflammatory activities.