Riaz Muhammad
International Islamic University, Pakistan
Title: Characterization and biomedical applications of silver nanoparticles synthesized by thermophilic bacteria.
Biography
Biography: Riaz Muhammad
Abstract
The biosynthesized Silver nanoparticles (AgNPs) are the most effective antimicrobial agents that are used in the medicine and pharmaceutics due to their outstanding optical, magnetic, electrical, catalytic and properties. The detectable antimicrobial action of silver bionanoparticles on many microorganisms are reported.
The main objective for undertaking the present study was to evaluate the extracellular synthesis of the AgNPs by natural thermophilic Bacillus Sps. AZ1 and AZ2 that were isolated from a hot spring (Tata Pani) in Kotli AJK. Afterwards the antimicrobial activity of the nanoparticle was evaluated against several human pathogenic organisms.
The biosynthesized AgNPs were confirmed visually by appearance of a dark brown color formation in the mixture as well as silver surface plasmon resonance band by using UV-Visible spectroscopy. The AgNPs were further characterized by SEM, EDX and TEM. The antimicrobial activity of the AgNPs was investigated using Salmonella typhi, Escherichia coli, Staphylococcus epidermis, and Staphylococcus aureus, by applying disk diffusion method.
Identification of the strains AZ1 and AZ2 by the 16S rRNA sequence analysis showed 99% sequence homology between Bacillus megaterium and Geobacillus stearothermophilus. The obtained UV-Visible spectrum of the aqueous medium containing silver ions, showed a peak at 435 nm which indicates a correspondence to the plasmon absorbance of the silver nanoparticles. The biosynthesized AgNPs were found to be in the size range of ~7-31 nm with spherical in shape.The antibacterial effect of the particles showed the highest inhibitory effect against the two strains; E. coli, and S. typhi, respectively.
The current study of biosynthesized silver nanoparticles offer a simple green synthesis process for the production of an extracellular nanoparticles which is environmental friendly. Biosynthesis of the AgNPs by a thermophilic bacillus Sps. from the hot spring (Tata Pani) in Kotli AJK with the highest similarity to Bacillusmegaterium and Geobacillus stearothermophilus are reported for the first time.