Anant Achary
Kamaraj College of Engineering and Technology, India
Title: Cytotoxic and antioxidant properties of Phlorotannin and fucoxanthin from Sargassum tenerrimum
Biography
Biography: Anant Achary
Abstract
hlorotannin and fucoxanthin from brown algae exhibit various beneficial biological activities. Sargassum tenerrimum , marine brown algae were collected from Gulf of Mannar, Mandapam Island, India. Solvent extraction of phlorotannin and fucoxanthin from S. tenerrimumwas was carried out using chloroform: methanol: water in the ratio 4:2:1. The presence of phenol and fucoxanthin in extracts were analyzed qualitatively by phytochemical screening and confirmed by FTIR analysis. Ferrous Reducing Antioxidant power (FRAP) of crude phlorotannin and fucoxanthin shows reducing power of 0.098 ± 0.095 and 0.00216 ± 0.00 (g of ferrous sulphate equivalent/g of sample ). The IC50 values for DPPH radical scavenging activity of phlorotannin was found to be 820.2 ± 36.7 and for fucoxanthin was found to be 726.75 ± 42.1 (µg/ml). Total antioxidant activity of crude phlorotannin and fucoxanthin was found to be 0.12 ± 0.002, 0.56 ± 0.028 g/of ascorbic acid equivalent/ g of sample), respectively.The results of total antioxidant power (TAP) assay, Ferrous Reducing antioxidant power (FRAP) assay and DPPH radical scavenging activity suggested that crude phlorotannin and fucoxanthinare had appreciable antioxidant activity. The cell proliferation assay using prostate cancer cell lines (PC3) showed that the IC50 value for phlorotannin and fucoxanthin was found to be 58±3.2 µg/mL and 60±2.8 (µg/mL) and the cytotoxicity is dose dependent. The phase contrast microscopic image of the phlorotannin and fucoxanthin treated PC3 cell lines showed shrinkage and other morphological abnormalities. The PC3 cells exhibited cellular DNA fragmentation when treated with the extracted phlorotannin and fucoxnthin and the DNA fragmentation pattern was found to be similar in the treated cells. Western blotting analysis revealed that phlorotannin and fucoxanthin suppressed the expression of BCL-2 (antiapoptotic protein) and enhanced the expression of Bax (proapoptotic protein) with β-actin as a control in treated and control PC3 cells. The present study suggests that phlorotannin and fucoxanthin from marine brown algae could be explored as potential chemotherapeutic agents for anticancer therapy