Rukhsana A Rub
Smt Kashibai Navale College of Pharmacy, India
Title: Identification and optimization of lead compound obtained from Celosia argentea
Biography
Biography: Rukhsana A Rub
Abstract
Background: An Indian origin, Celosia argentea is a weed growing during rainy season, traditionally claimed for treating several ailments. Early researches on C. argentea were focused on the anti-cancer screening of seeds, with few reports on aerial parts.
Objective: To isolate, characterize and evaluate drug like potential of bioactive compound obtained from aerial parts of C. argentea.
Methodology: The methanolic aerial part extract was fractionated and purified on MCI-HP20 HPLC Column. Chromatographically pure compounds were pooled, concentrated, screened for cytotoxic potential and were characterized spectroscopically. The elucidated active molecule was further subjected to elaborate drug likeness study.
Results: The isolated bioactive phenolic compound was confirmed as, 1-(4-hydroxy- 2-methoxybenzofuran-5-yl)-3- phenylpropane-1,3-dione which showed potent cytotoxicity against SiHa, HCT and MCF-7 cancer cell lines at 18 μg/ml (**P< 0.01), without affecting the normal Vero cells. Apoptosis occurred at concentrations of 35.15 ± 0.4 μg/ml and 28.05 ± 0.3 μg/ ml for HT-29 and MCF-7 respectively. The ADME, toxicity and drug likeness study indicated that the drug is nontoxic, better absorbed through intestine, does not cross BBB and qualifies Lipinski’s, CMC like, MDDR,WDR like rules.
Conclusion: A novel anticancer phenolic compound; (1-(4-hydroxy-2 methoxybenzofuran-5-yl)-3-phenylpropane- 1,3-dione can be regarded as a potential anticancer drug molecule.